rabbit anti human cd10  (Bioss)

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Standalone methacrylated extracellular matrix for digital light processing bioprinting: a practical workflow

doi: 10.3389/fbioe.2026.1774476

Figure Lengend Snippet: 3D bioprinted dECM-MA bioink supports high-density parenchymal cell attachment, and early ECM remodeling. Human uterine stromal fibroblast cells (primary parenchymal cells of the uterine tissue, FC-0076 Lifeline Cell Technologies) were cultured in commercial media, exhibiting characteristic spindle-shaped stromal morphology (A) . These cells were immunofluorescently characterized for positive uterine and stromal markers (CD10, CD73, and Vimentin, respectively). CD31 isotype staining served as a negative control. Following expansion, cells were seeded at high density onto 3D bioprinted dECM-MA scaffolds, cultured for 16 h, and characterized by HR-SEM (B) . An overview SEM image shows a visibly high cell density on the ECM-MA after culture. Higher magnifications reveal a high-density (yellow rectangle, zoomed in) and lower density (blue rectangle, zoomed in) areas where human uterine stromal cells (yellow arrow) interact with the dECM-MA fibers (green arrow), suggesting biological interaction and constructive remodeling.

Article Snippet: Specifically, rabbit anti-human CD10 (Bioss, Cat. No. BS-0527R-20; RID: AB_10854297) and rabbit anti-human vimentin (Bioss, Cat. No. BS-0756R-20; RRID: AB_10855343) were used as primary antibodies, with a rabbit IgG isotype control (Santa Cruz Biotechnology, Cat. No. sc-8306; RRID: AB_653100).

Techniques: Cell Attachment Assay, Cell Culture, Staining, Negative Control

Journal: Experimental and Therapeutic Medicine

Article Title: A novel nude mouse model for studying the pathogenesis of endometriosis

doi: 10.3892/etm.2022.11425

Figure Lengend Snippet: Immunofluorescence characterization of the endometriosis-like lesions. (A and B) Glandular epithelial cells were intensely stained for E-cadherin and surrounding stromal cells were mildly stained for CD10. (C and D) Glandular epithelial cells were intensely stained for cytokeratin 7 and surrounding stromal cells were mildly stained for vimentin.

Article Snippet: The primary antibodies including mouse anti-cytokeratin 7 (1:500 dilution, 66483-1, ProteinTech Group, Inc.), mouse anti-E-cadherin (1:500 dilution, ab40772, Abcam), rabbit anti-human CD10 antibodies (1:250 dilution, 18008-1-AP, ProteinTech Group, Inc.) and rabbit anti-human vimentin (1:500 dilution, ab45939, Abcam) were used to identify stromal and epithelial cells and incubated overnight at 4 ̊C.

Techniques: Immunofluorescence, Staining